What is the function of LEF?
What is the function of LEF?
TCF/LEF can activate transcription of downstream target genes upon activation by a variety of upstream signals and regulate biological activities, such as differentiation, proliferation, and apoptosis of osteoblasts. These play an important role in bone development, growth, and remodeling.
Does beta catenin bind to DNA?
Nuclear β-catenin associates with DNA-binding transcription factors of the TCF/Lef family. The binding of β-catenin converts TCF/Lef proteins into bipartite TCF/β-catenin transcriptional activators, converting the Wnt signal into the transcription of specific target genes (Najdi et al, 2011; Archbold et al, 2012).
Is Wnt a growth factor?
The Wnt family of secreted growth factors plays key roles in directing cell patterning both during development and in adult tissues. Consequently, it is not surprising that signalling pathways downstream of Wnts have been implicated in oncogenesis.
How does the Wnt pathway work?
The Wnt signaling pathways are a group of signal transduction pathways which begin with proteins that pass signals into a cell through cell surface receptors. The canonical Wnt pathway leads to regulation of gene transcription, and is thought to be negatively regulated in part by the SPATS1 gene.
What type of protein is B catenin?
Catenin beta-1, also known as β-catenin, is a protein that in humans is encoded by the CTNNB1 gene. β-catenin is a dual function protein, involved in regulation and coordination of cell–cell adhesion and gene transcription. In humans, the CTNNB1 protein is encoded by the CTNNB1 gene.
What is the role of the TCF / LEF family?
TCF/LEF family. The TCF/LEF family is a group of transcription factors which bind to DNA through a SOX-like high mobility group domain. They are involved in the Wnt signaling pathway, where they recruit the coactivator beta-catenin to enhancer elements of genes they target. They can also recruit members of the Groucho family of corepressors.
How are TCF / LEF factors involved in Wnt signaling?
TCF/LEF factors regulate G-CSF receptor expression by directly interacting with CSF3R promoter and enhancer regions. The canonical Wnt signaling pathway is mediated by interaction of β-catenin with the T-cell factor/lymphoid enhancer-binding factor (TCF/LEF) transcription factors and subsequent transcription activation of Wnt-target genes.
Where is the luciferase gene located in the TCF / LEF reporter?
This reporter contains a firefly luciferase gene under the control of multimerized TCF/LEF responsive element located upstream of a minimal promoter. The TCF/LEF reporter is premixed with constitutively-expressing Renilla luciferase vector that serves as internal control for transfection efficiency.
How does the TCF / LEF reporter kit work?
The TCF/LEF reporter is premixed with constitutively-expressing Renilla luciferase vector that serves as internal control for transfection efficiency. The kit also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as negative control.